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Key findings with the 1.6 protocol included: (1) VO2max was higher than for the 1.2 protocol and appeared to be unaffected by age ( P = 0.370); (2) HRmax was lower than VO2max values for 1.2 and 1.6 protocols ( P = 0.019); (3) Aerobic capacity measured in terms of VO2peak increased with age ( P < 0.001) and did not differ between the protocols in our cohort; (4) There was no difference in VO2peak between protocols ( P = 0.447); and (5) VO2peak did not vary significantly between sessions for each age group ( P = 0.145).For cardiovascular measures the present study shows that HR was well correlated to running velocity for each protocol (Fig. 6A, R2 = 0.788, P = 0.004, n = 74) ( Fig. 7A, R2 = 0.656, P < 0.001, n = 74). Therefore, following the calculations that assume a fixed HR/VO2max relationship, VE/VO2 for both protocols were similar and VE/VO2 for each protocol was higher than the average values reported for mice by others for mice and rats (20, 41, 47, 48), demonstrating the close adherence of the present protocols with other murine studies.At the end of the 6 minute protocol, the participant had a marked decrease in HR from the minimum HR determined at the start of the protocol to a resting HR. As the protocol continued, the HR decreased further such that within 12 minutes the participant attained a HR of 86% of the peak HR attained during the 6 minute protocol. This finding, that a marked decrease in HR occurs early in the protocol suggests the protocol is sufficiently demanding to elicit a maximal effort even before the protocol 6 minute time point.
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