The final concentration would be: Tris,mmol/L; glycine, mmol/L; SDS, % and the pH of the buffer is Polyacrylamide gel electrophoresis tank ml% SDS Stacking gel master mixml H2O ml M Tris pHml% SDS Pouring resolving gelMakeml of resolving gel (makesgel, with a little bit leftover) ml of resolving gel master mix ml of% acrylamideμl of% APS μl of TEMEDImmediately load gel mixture into the casing with a pipette SectionGeneral InformationIntroduction The Mini-PROTEAN. Dilutefold when using. Scientific, Inc. makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto An SDS is an important document that explains the hazards, precau-tions, and response acions for a particular chemical-containing product. Safety Data Sheet SDSRevision Date: This Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. Part IV – Western BlotPrepare fresh 1X Transfer Buffer (TGM) ®Tetra cell runs both handcast gels and Ready Gel®precast gels interchangeably. All washes are at RTPlace gel overpieces of 3mm paper, cover with Saranwrap, and dry in gel dryer for h atºC. SDSs are developed by the manufacturer and provided by the product supplier Tris glycine electrophoresis buffer: weigh g Tris andg glycine; Dissolve in ml deionized water; then addml% (w/v) SDS and deionized water to ml. An SDS also explains proper medical or first aid treatment, should overexposure occur. The Mini-PROTEAN Tetra system includes a casting stand and glass plates with permanently bonded gel spacers that simplify handcasting and eliminate leaking during castingWash with Destaining Solution(5% MeOH, 7% HAc) a few times, with the final wash being done overnight.